Bifidogenic peptides

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai

Reexamination Certificate

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C514S012200, C514S014800, C514S018700, C435S252800, C435S254100, C435S255100, C530S324000, C530S327000, C530S360000, C530S361000, C530S365000, C530S366000

Reexamination Certificate

active

06849593

ABSTRACT:
Pepetides having bifidogenic properties are obtainable by the process of adding proteases to cow'milk or human milk, followed by incubation, centrifugation, acidification, purification by reverse phase HPLC and cation-exchange HPLC, culturingBifidobacterium bifidumandE. coliin the presence of collected bifidogenic fractions, and isolation of the peptides having bifidogenic properties, and the isolated peptides can be amidated, acetylated, sulfated, phosphorylated, glycosylated, oxidized, or fragmented and still maintain their bifidogenic properties, and combination peptides having bifidogenic properties are obtainable by chemically bonding the peptides having bifidogenic properties, the amidated, acetylated, sulfated, phosphorylated, glycosylated, oxidized, or fragmented peptides having bifidogenic properties, or combinations thereof.

REFERENCES:
patent: WO 9013642 (1990-11-01), None
patent: WO-9608269 (1996-03-01), None
Voet et al., Biochemistry, pp. 112-113 John Wieley & Sons, 1990.*
Wayne Bellamy, “Identification of the Bactercidal Domain of Lactoferrin”, pp. 130-136 Biochimica Et Biophysica Acta, NL, Amsterdam (1992).
Wayne Bellamy, “Antibacterial Spectrum of Lactoferricin B, a Potent Bactericidal Peptide Derived from the—Terminal Region of Bovine Lactoferrin”, pp. 472-479, Journal of Bacteriology, US, Washington, DC, (1992).
Ametani, E.A.: “Antibody Response of Three Different Strains of Mice to Alpha-S1-Casein Analyzed by Using Proteolytic and Synthetic Peptides”,Biochemical and Biophysical Research Communications, vol. 154, No. 3 (Aug. 15, 1988), pp. 876-882.
Spuergin, P. et al.: Allergenic Epitopes of Bovine.Alpha.S1-Casein Recognized by Human IgE and IgG,Allergy(Copenhagen) (1996), 51 (5), 306-312 Coden: LLRGDY: 0105-4538, 1996.
Database WPI, Section Ch, Week 9439, Derwent Publications Ltd., London, GB; An 94-313708 & JP 06 239 888 A (Kanebo Ltd.) (Aug. 30, 1994), see abstract.
Kizawa: “Calmodulin Binding Peptide Comprising Alpha-Casein Exorphin”,J.Agric.Food.Chem., vol. 45, No. 5, May 1997, pps. 1579-1581.
Bellamy, E.A.: “Antibacterial Spectrum of Lactoferricin b, A Potent Bactericidal Peptide Derived From the N-Terminal Region of Bovine Lactoferrin”,J. Applied Bacteriology, vol. 73, No. 6, 1992, pps. 472-479.
Etienne E.A.: “Growth Promotion of Bifidobacterium Animalis by Bovine Milk Proteose-Peptone”,LAIT, vol. 74, 1994, pps. 313-323.
Proulx, E.A.: “Comparison of Bifidobacterial Growth-Promoting Activity of Ultrafiltered Casein Hydrolyzate Reactions”,LAIT, vol. 74, 1994, pps. 139-152.

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