Bidentate conjugate and method of use thereof

Details Bidentate conjugate and method of use thereof Bidentate conjugate and method of use thereof

1992-07-31

1995-06-06

Kim, Kay K. A.

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

436517, 436536, 436815, 436816, 436817, G01N 3353, G01N 33536, G01N 33566

Patent

active

054222818

ABSTRACT:
A novel bidentate conjugate has two different chemical moieties, or bidentate members, attached through an adequate spacer moiety. Each bidentate member acts as a small molecule ligand and is capable of specifically binding to a different macromolecular substance. The bidentate members are relatively small molecules, usually less than about 7,000 Daltons in size. The spacer moiety is selected so that both bidentate members can simultaneously bind to their respective specific binding partners. Where the specific binding partners are multivalent, large complexes can be formed. The formation of these complexes can be inhibited by the presence of an unconjugated monovalent bidentate member, such as free analyte from a test sample. The bidentate is of particular use in turbidimetric or nephelometric inhibition immunoassay procedures.

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