Bicarbonate assay and phosphoenolpyruvate carboxylase enzyme fro

Details Bicarbonate assay and phosphoenolpyruvate carboxylase enzyme fro Bicarbonate assay and phosphoenolpyruvate carboxylase enzyme fro

1993-03-30

1996-05-07

Gitomer, Ralph J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 15, 435 26, 435975, C12Q 100, C12Q 148, C12Q 132

Patent

active

055145388

ABSTRACT:
Phosphoenolpyruvate carboxylase (PEPC) characterized by a specific activity of at least 40 units/mg; and/or by no more than 50% loss of activity after 60 days in 167 mM Tricine/HCl buffer, pH 8.0, and 15 mM MgCl.sub.2 ; and/or, at pH 6.5, an activity of at least 40% relative to the activity at optimum pH is disclosed. Such enzymatic activity can be obtained from serine pathway methylotrophs such as Hyphomicorbium, and is very suitable for use in measuring bicarbonate in biological samples.

REFERENCES:
patent: 5112740 (1992-05-01), Nealon
patent: 5116728 (1992-05-01), Crowther
Doronina, N. V., The Properties of a New Hyphomicrobium Microbiologiya, 54 (4) 1985 pp. 538-544. (English).
Plant Physiology, vol. 57, 1976, pp. 906-910 "Purification & Characterization of PEPC from Maize Leaves".
Applied And Environmental Microbiology, vol. 40, No. 2, Aug. 1980, pp. 370-375. "Regulation of Enzymes Assoc. w C-1 Metabolism in 3 Facultative Methylotrophs".

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