Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2011-08-09
2011-08-09
Fronda, Christian (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S183000, C435S201000, C536S023200
Reexamination Certificate
active
07993884
ABSTRACT:
Xylose-containing plant material may be hydrolyzed to xylose using a β-D-xylosidase which exhibits unexpectedly high activity. The enzyme has a kcatvalue for catalysis of approximately 185 sec−1for 1,4-β-D-xylobiose (X2) when measured at a pH of 5.3 and a temperature of 25° C.; this is at least 10-fold greater than reported for other xylosidases at 25° C. and their optimal pH. The enzyme also has an isoelectric point of approximately 4.4. When reacted at a pH between about 4.5 and about 7.7, the β-D-xylosidase exhibits surprisingly high activity for hydrolyzing xylose-containing plant materials to xylose. The xylose released from plant materials may then be converted to other secondary products such as ethanol by fermentation or other reaction. This β-D-xylosidase may be used alone or in combination with other hydrolytic or xylanolytic enzymes for treatment of lignocellulosic or hemicellulosic plant materials or plant material hydrolysates or xylooligosaccharides.
REFERENCES:
Chica et al. Curr Opin Biotechnol. Aug. 2005;16(4):378-84.
Sen et al. Appl Biochem Biotechnol. Dec. 2007;143(3):212-23.
Accession O52575. Jun. 1, 1998.
Saha. J Ind Microbiol Biotechnol. May 2003;30(5):279-91. Epub Apr. 16, 2003.
Cotta Michael A.
Dunlap Christopher A.
Jordan Douglas B.
Li Xin Liang
Whitehead Terence R.
Deck Randall E.
Fado John
Fronda Christian
Shaw Lesley
The United States of America as represented by the Secretary of
LandOfFree
Beta-xylosidase for conversion of plant cell wall... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Beta-xylosidase for conversion of plant cell wall..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Beta-xylosidase for conversion of plant cell wall... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2726844