Beta-lactam substrates and uses thereof

Details Beta-lactam substrates and uses thereof Beta-lactam substrates and uses thereof

1998-07-23

2000-02-29

Trinh, Ba K.

Organic compounds -- part of the class 532-570 series

Organic compounds

Unsubstituted hydrocarbyl chain between the ring and the -c-...

540364, 435 18, 435 19, C07D205085, C07D20508

Patent

active

060310944

ABSTRACT:
##STR1## Substrates for beta-lactamase of general formula 1, in which R.sup.1 is a moiety, in which a primary amine attached to an aryl carbon of a fluorophore precursor was converted into the nitrogen atom of the beta-lactam ring and the fluorophore precursor is not anthranilic acid when R.sup.2 is H; R.sup.2 is selected from the group consisting of H and --NR.sup.3 R.sup.4, in which R.sup.3 and R.sup.4 are selected from the group consisting of H, aliphatic, alkyl, and acyl; and the beta-lactam ring may be cleaved by a beta-lactamase enzyme. Methods of assaying beta-lactamase activity and monitoring expression in systems using beta-lactamase as a reporter gene are also disclosed.

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