.beta.-fructofuranosidase, its preparation and uses

Details .beta.-fructofuranosidase, its preparation and uses .beta.-fructofuranosidase, its preparation and uses

1996-12-11

1998-05-19

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 96, 435 97, 435100, 435101, 435200, 435201, C12P 1914, C12P 1920, C12N 924, C12N 926

Patent

active

057534691

ABSTRACT:
A .beta.-fructofuranosidase with a molecular weight of 49,000.+-.5,000 daltons on SDS-PAGE, an isoelectric point of 4.6.+-.0.5, an optimum pH of about 5.5-6.0, and an optimum temperature of about 50.degree. C. in the presence of calcium ion. The enzyme acts on saccharides with a .beta.-fructofuranosidic linkage and other substances including other saccharides, sugar alcohols, and alcohols to produce fructosyl-transferred saccharides in a relatively high yield.

REFERENCES:
patent: 4487198 (1984-12-01), Miyake et al.
patent: 4521252 (1985-06-01), Miyake et al.
Patent Abstracts of Japan, vol. 016, No. 528, 29 Oct. 1992 (JP 04 200386 A).
Sneath, Peter H. ed. et al., "Bergey's manual of systematic bacteriology" vol. 2, Williams & Wilkins, 1986.
Fujita, Koki et al., "Purification and some properties of B-Fructofuranosidase I from arthrobacter sp. K-1.", Agric. Biol. Chem., vol. 54, No. 4, pp. 913-919 (1990).
Hasegawa, Takeji ed., "Classification and identification of microorganisms." Japan Scientific Societies Press, (1985).

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