.beta.-fructofuranosidase its preparation and uses

Details .beta.-fructofuranosidase its preparation and uses .beta.-fructofuranosidase its preparation and uses

1997-09-30

1998-11-17

Prats, Francisco C.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435832, 435837, 435200, 435201, 435 96, 435 97, 435 99, 435100, 435101, C12N 120, C12N 926, C12P 1914

Patent

active

058375279

ABSTRACT:
A .beta.-fructofuranosidase with a molecular weight of 49,000.+-.5,000 daltons on SDS-PAGE, an isoelectric point of 4.6.+-.0.5, an optimum pH of about 5.5-6.0, and an optimum temperature of about 50.degree. C. in the presence of calcium ion. The enzyme acts on saccharides with a .beta.-fructofuranosidic linkage and other substances including other saccharides, sugar alcohols, and alcohols to produce fructosyl-transferred saccharides in a relatively high yield.

REFERENCES:
patent: 4487198 (1984-12-01), Miyake et al.
patent: 4521252 (1985-06-01), Miyake et al.
Sneath, Peter H. ed. et al., "Bergey's manual of systematic bacteriology" vol. 2, Williams & Wilkins, 1986.
Fujita, Koki et al., "Purification and some properties of .beta.-Fructofuranosidase I from arthrobacter sp. K-1.", Agric. Biol. Chem., vol.54, No. 4, pp. 913-919 (1990).
Hasegawa, Takeji ed., "Classification and identification of microorganism.", Japan Scientific Societies Press, (1985).
Patent Abstracts of Japan, vol. 016, No. 528, 29 Oct. 1992 (JP 04 200386 A) .

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