.beta.-Endotoxin expression in pseudomonas fluorescens

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 697, 43575234, 435 3201, 424405, 424538, 514 2, 530350, 536 234, 536 2371, C12N 1511, C12N 1532, C12N 1578, A01N 6300

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058405542

ABSTRACT:
Bacillus thuringiensis endotoxin expression in Pseudomonads can be improved by modifying the gene encoding the Bacillus thuringiensis endotoxin. Chimeric genes are created by replacing the segment of the Bacillus thuringiensis gene encoding a native protoxin with a segment encoding a different protoxin. Exemplified herein is the cryIF/cryI(b) chimera wherein the native cryIF protoxin segment has been substituted by the cryIA(b) protoxin segment, to yield improved expression of the cryIF toxin in Pseudomonads. The invention also concerns novel genes and plasmids.

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