.beta.-Carotene producing strains of the fungus phycomyces blake

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 67, 435254, 435911, C12N 1500, C12P 2300, C12N 114, C12R 1645

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active

043189873

ABSTRACT:
This invention refers to the obtention of strains of the fungus Phycomyces blakesleeanus accumulated by the .beta.-carotene pigments in amounts which permit the use of these strains in the production on an industrial scale of said pigment. By genetic manipulations, strains simultaneously and constitutively incorporating various stimulating effects have been isolated. The resulting strains produce .beta.-carotene in a proportion of approximately 2.5% of its dry weight, which constitutes almost 1000 times more than that which the natural microorganism would produce under the same conditions. This production level, together with the simplicity in cultivating and manipulating same, makes them interesting in the development of an industrial process for producing .beta.-carotene by fermentation. The .beta.-carotene produced by fermentation, besides its low cost, would have the advantage of not being contaminated with the reagents and catalysts used in the organic synthesis.

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Cerda-Olmedo et al., "Genetic Classification of the Lethal Effects of Various Agents on Heterokaryotic Spores of Phycomyces", Mutation Res., vol. 9, (1970), pp. 369-384.
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