.beta.-amylase gene

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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4352523, 43525231, 4353201, 536 27, C12N 926, C12N 1556, C12N 1575, C12N 121, C12N 100

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active

050827812

ABSTRACT:
A novel .beta.-amylase gene is provided. This gene is useful for production of a thermophilic .beta.-amylase obtained from Clostridium thermosulfurogenes on a large scale, especially from genetically engineered microorganisms introduced with this gene. B. subtilis was transformed with a plasmid pNK1 containing the above gene and the transformed B. subtilis cells produced .beta.-amylase having the same thermostability as that of .beta.-amylase obtained from C. thermosulfurogenes.

REFERENCES:
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patent: 4529695 (1985-07-01), Weisblum
patent: 4647538 (1987-03-01), Zeikus et al.
Biosis Database, Abstract No. 87037025, N. Kitamo et al., "Cloning and Sequencing of the Gene Encoding Thermophilic Beta Amylase of Clostridium-Thermosulfurogenes" & J. of Bacteriology (US), vol. 170, No. 12, 1988, pp. 5848-5854.
Biosis Database, Abstract No. 35038111, L. Bhatnagar et al., "Cloning of Beta Amylase From Clostridium-Thermosulfurogenes Strain 4B", & Abstracts of the Annual Meeting of the American Society for Microbiology, vol. 88, No. 0, 1988, p. 211.
Chemical Abstracts, vol. 103, No. 1, Jul. 8, 1985, Abstract No. 2663n, Columbus, Ohio, H. H. Hyun et al., "General Biochemical Characterization of Thermostable Extracellural . . . " & Appl. Environ. Microbiol., vol. 49, No. 5, 1989, pp. 1162-1167.
Chemical Abstracts, vol. 108, No. 5, Feb. 1, 1988, Abstract No. 36267e, Columbus, Ohio, Badal C. Saha et al., "Behavior of A Novel Thermostable Beta-Amylase on Raw Starch" & Enzyme Microb. Technol., vol. 9, No. 10, 1987, pp. 598-601.
Chemical Abstracts, vol. 109, No. 19, Nov. 7, 1988, Abstract No. 166194k, Columbus, Ohio, Gwo Jenn Shen et al., "Purification and Characterization of A Novel Thermostable Beta-Amylase From Clostridium . . . ", vol. 254, No. 3, 1988, pp. 835-840.

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