.beta.-Amylase determination

Details .beta.-Amylase determination .beta.-Amylase determination

1980-05-07

1985-10-29

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 14, 435 18, 435 26, 435 28, 435202, 435203, 435204, 435 99, 435810, C12Q 140, C12Q 132, C12Q 128, C12P 1914

Patent

active

045500770

ABSTRACT:
A reagent suitable for .alpha.-amylase determinations. The reagent contains a modified, blocked amylaceous polysaccharide substrate, e.g., starch glyolate, an exating by amylose, a buffer and an enzymatic glucose measuring system. The glycolate substrate is selected such that the ratio of methylated glucose to glucose is between 1:4 to 1:16. The system permits an impid assay in a single vid within 7 minutes and can be automated.

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Huang, et al., "A Specific Sensitive Assay for .alpha.-Amylase Utilizing Modified Starch as Substrate", Clin. Chem., vol. 22, No. 7 (1976), p. 1164.
Nix, et al., "A New Method for Determination of .alpha.-Amylase, Clin. Chem., vol. 24, No. 6, (1978), p. 1000.
Madappally et al., "Enzymatic Determination of Serum Amylase," Clin. Chem., vol. 22, No. 7, (1976), p. 1000
Clinica Chimica Acta, 76, (1977), pp. 277-283, by J. John Marshall et al.

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