Beta-alanine/alpha-ketoglutarate aminotransferase for...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Reexamination Certificate

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C435S183000, C435S135000, C435S141000, C435S252300, C435S254110, C435S254200, C435S320100, C435S069100, C435S091100, C536S023200, C536S023100

Reexamination Certificate

active

08030045

ABSTRACT:
The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof.

REFERENCES:
patent: 7630836 (2009-12-01), Omura et al.
patent: WO 2005/118719 (2005-12-01), None
patent: WO2006069610 (2006-07-01), None
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Database UniProt [Online] Accession No. Q82ER2, “Putative Aminotransferase,” Jun. 1, 2003 and Database EMBL [Online] Accession No. BA000030, “Streptomyces avermitilisMA-4680 Genomic DNA, Complete Genome,” Oct. 31, 2004.
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Database UniProt [Online] Accession No. Q05881, “4-Aminobutyrate Transaminase (EC 2.6.1.19),” Sep. 5, 2006 and Database EMBL [Online] Accession No. CP000431, “Rhodococcussp. RHA1, Complete Genome,” Aug. 7, 2006.
Ikeda et al., “Complete Genome Sequence and Comparative Analysis of the Industrial MicroorganismStreptomyces avermitilis,” Nature Biotechnol. 21:526-531, 2003.
McLeod et al., “The Complete Genome ofRhodococcussp. RHA1 Provides Insights into a Catabolic Powerhouse,”Proc. Natl. Acad. Sci. USA 103:15582-15587, 2006.
Yonaha et al., “Distribution of Omega Amino-Acid Pyruvate Transaminase EC-2.6.1.18 and Amino Butyrate Alpha Keto Glutarate Transaminase EC-2.6.1.19 in Microorganisms,”Agricult. Biol. Chem. 47:2257-2266, 1983.

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