Beads for capturing target cells from bodily fluid

Details Beads for capturing target cells from bodily fluid Beads for capturing target cells from bodily fluid

2004-12-23

2008-10-21

Naff, David M (Department: 1657)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C435S029000, C435S177000, C435S180000, C435S181000

Reexamination Certificate

active

07439062

ABSTRACT:
Spheroidal beads present an exterior surface of a hydrophilic hydrogel, which is an isocyanate-functional polymer that is polymerized by urethane bonds and cross-linked by urethane and urea bonds. Sequestering agents present at the surface are covalently bound to isocyanate groups or to intermediate linkers that are so bound. These beads allow sequestering agents to retain their native three-dimensional configuration, and as a result of such surface characteristics and hydrophilicity, they achieve highly effective capture of very small subpopulations of rare cells from bodily fluids or the like and very effectively deter nonspecific binding of other biomaterials present in such bodily fluid. They may be all-hydrogel spheroids or hydrogel-coated substrates.

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