Bead linkers for immobilizing nucleic acids to solid supports

Details Bead linkers for immobilizing nucleic acids to solid supports Bead linkers for immobilizing nucleic acids to solid supports

1996-11-06

1999-05-04

Kunz, Gary L.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 56, 5361231, 502233, 5253322, C07H 100, C07H 2104, C07H 3318

Patent

active

059004819

ABSTRACT:
Novel compositions comprised of at least one bead conjugated to a solid support and further conjugated to at least one nucleic acid and preferred methods for making the novel compositions are described. As compared to "flat" surfaces, beads linked to a solid support provide an increased surface area for immobilization of nucleic acids. Furthermore, by selecting a bead with the desired functionality, a practitioner can select a functionalization chemistry for immobilizing nucleic acids, which is different from the chemistry of the solid support.

REFERENCES:
patent: 4139346 (1979-02-01), Rabbani
patent: 4797355 (1989-01-01), Stabinsky
patent: 4806546 (1989-02-01), Carrico et al.
patent: 5242974 (1993-09-01), Holmes
patent: 5380833 (1995-01-01), Urdea
patent: 5512439 (1996-04-01), Hornes et al.
Arshady, Reza; Review: Beaded Polymer Supports and Gels, I. Manufacturing Techniques; Journal of Chromatography, 586 (1991); pp. 181-197.
Arshady, Reza; Review: Beaded Polymer Supports and Gels, II. Physico-Chemical Criteria and Functionalization; Journal of Chromatography, 586 (1991); pp. 199-219.
Damha, Masad J. et al.; An Improved Procedure for Derivatization of Controlled-Pore Glass Beads for Solid-Phase Oligonucleotide Synthesis; Nucleic Acids Research vol. 18, No. 13 (1990); pp. 3813-3821.
Hayashi, Toshio et al.; Immobilization of Thiol Proteases onto Porous Poly(Vinyl Alcohol) Beads; Polymer Journal vol. 25, No. 5 (1993); pp. 489-497.
Lund, Vera et al.; Assessment of Methods for Covalent Binding of Nucleic Acids to Magnetic Beads, Dynabeads, and the Characteristics of the Bound Nucleic Acids in Hybridization Reactions; Nucleic Acids Research vol. 16, No. 22 (1988).
Pon, Richard T. et al.; Research Report: Derivatization of Controlled Pore Glass Beads for Solid Phase Oligonucleotide Synthesis; BioTechniques vol. 6, No. 8 (1988); pp. 768-770, 773-775.

Affiliated with

Also associated with

Rating

Bead linkers for immobilizing nucleic acids to solid supports does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Bead linkers for immobilizing nucleic acids to solid supports, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Bead linkers for immobilizing nucleic acids to solid supports will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1869984