Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-11-02
1996-03-19
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 2, 436513, 436518, 436526, 436531, 436548, 5303887, G01N 33543, G01N 33577
Patent
active
055003486
ABSTRACT:
The monoclonal antibodies of the present invention makes it possible to separate basophils suitable for the IgE-mediated specific chemical mediator release test, because it retains its reactivity with basophils even after being immobilized onto a solid carrier, and because it does not inhibit release of chemical mediators induced by allergens or anti-IgE antibody, and does not induce nonspecific release of chemical mediators. Also, the method for separating basophils of the present invention simplifies the separation of basophils from blood, and by using this method, the histamine release test which otherwise requires complex procedures can be simplified. Further, the group of cells obtained by the method for separating basophils of the present invention can easily be utilized in the release tests for chemical mediators released from basophils such as leukotriene and PAF, which otherwise require expertise for handling.
REFERENCES:
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Nishi Hiroshi
Nishimura Masaji
Nishimura Shinji
Grun James L.
Scheiner Toni R.
Shionogi & Co. Ltd.
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