Base sequencing apparatus

Details Base sequencing apparatus Base sequencing apparatus

1993-05-24

1995-11-21

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041828, G01N 2726, G01N 27447

Patent

active

054683645

ABSTRACT:
Disclosed herein is an on-line base sequencing apparatus, which is provided with electrophoresis tubes having a sheath flow part in each lower end thereof, which a sulfuric acid solution of pH 1.0 is continuously fed to form a sheath flow. The portions close to the outlets provided in the lower ends are irradiated with light which is close to 260 nm. In order to detect fluorescence components, each electrophoresis tube is provided with a detection system involving an interference filer for selecting that of 340 to 390 nm from the fluorescence components. Outputs of such four detection systems are successively identified, to determine base sequences.

REFERENCES:
Robert E. Milofsky and Edward S. Yeung "Native Fluorescence Detection of Nucleic Acids and DNA Restriction Fragments in Capillary Electrophoresis" Analytical Chemistry (Jan. 1993) 153-157.
Norman J. Dovichi et al "Three DNA Sequencing Methods Using Capillary Gel Electrophoresis and Laser Induced-Fluorescence" Analytical Chemistry (Dec. 1991) 2835-2841.
Thomas T. Lee and Edward S. Yeung "High-sensitivity laser-induced fluorescence detection of native proteins in capillary electrophoresis" Journal of Chromatography 595 (1992) 319-325.

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