Base sequencing apparatus

Details Base sequencing apparatus Base sequencing apparatus

1992-10-15

1995-05-30

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041828, 36441301, 435 6, C25B 700, C25B 900

Patent

active

054198259

ABSTRACT:
An on-line system base sequencing apparatus wherein calibration coefficients for time bases of respective electrophoresis lanes are evaluated from differences between positions of signals already outputted in a range causing no sequence inversion and positions of substantially regular intervals for originally outputting signals, and time bases as to the respective electrophoresis lanes are calibrated with the calibration coefficients, thereby obtaining correct base sequence. Thus, the bases can be correctly sequenced even if electrophoresis speed differences are caused between the electrophoresis lanes.

REFERENCES:
patent: 4720786 (1989-01-01), Hara
Lance B. Koutny and Edward S. Yeung, "Automated Image Analysis for Distortion Compensation in Sequencing Gel Electrophoresis" Applied Spectroscopy, vol. 46, No. 1 (Jan. 1992) 136-141.
Philip Taylor et al "Computer assisted size measurement on digitising tablet of nucleic acid and protein molecules from gels" Journal of Biochemical and Biophysical Methods, 14 (1987) 71-83.

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