Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1989-06-13
1992-06-16
Fox, David T.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 691, 435 701, 43524045, 43524049, 4353201, 935 52, 935 56, 935 67, C12N 1500, C12N 500, C12P 2100, C12P 2104
Patent
active
051224666
ABSTRACT:
A method of transforming conifers with a DNA construct comprising an expression cassette is disclosed herein. The method comprises the steps of (a) providing a plant tissue target, the plant tissue target comprised of conifer tissue, the cells of the conifer tissue including cell walls; and then (b) propelling the DNA construct at the plant tissue target at a velocity sufficient to pierce the cell walls and deposit the DNA construct within a cell of the tissue target to thereby provide a transformed plant tissue. Preferably, shoots are then induced to form on the transformed plant tissue. Preferred plants for carrying out the present invention are the members of the genus Pinus.
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Sederoff Ronald R.
Stomp Anne-Marie
Weissinger Arthur K.
Fox David T.
North Carolina State University
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