Baculovirus vector, method of producing thereof and method...

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

Reexamination Certificate

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C435S069100, C435S455000, C435S320100

Reexamination Certificate

active

07416890

ABSTRACT:
The present invention provides a novel baculovirus vector which can provide a desired protein not requiring infectivity to insect cells, on the viral particle surface; method of producing thereof; and method of gene transfer using the baculovirus vector. A method of producing a baculovirus vector including; a process of cotransfecting at least a plasmid containing a gene coding a protein capable of being expressed on a cell surface and one of wild-type, mutated-type form, and recombinant baculovirus DNAs into insect cells, wherein a pseudotyped baculovirus which includes at least one part of the baculovirus DNA and is coated with the protein capable of being expressed on a cell surface, is generated.

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