Baculovirus expression system for human interleukin 5 receptor a

Details Baculovirus expression system for human interleukin 5 receptor a Baculovirus expression system for human interleukin 5 receptor a

1997-01-11

1999-01-19

Draper, Garnette D.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435348, 4353201, 536 235, 935 23, 935 57, 935 70, C12N 1587, C12N 1563

Patent

active

058612793

ABSTRACT:
A baculovirus/insect cell expression system in which both .alpha. and .beta. subunit of human IL-5 receptor can be over-expressed together in Sf9 cells has been developed. This system is able to express the human high-affinity IL-5 receptors at a very high level and finds use in a screening assay for IL-5 receptor-based antagonists. A preferred embodiment of the screening assay involves the use of peanut agglutinin as lectin in a scintillation proximity assay.

REFERENCES:
patent: 4568649 (1986-02-01), Bertoglio-Matte
patent: 5112961 (1992-05-01), Hayashida et al.
patent: 5229501 (1993-07-01), Keifer et al.
patent: 5453491 (1995-09-01), Takatsu et al.
Blanco et al., Proc. Natl. Acad. Sci. USA, 90(5):1824-1828 (1993).
Cascio, Meth. Neurosci., 25:175-200 (1995).
Coffman et al., Science, 245:308-310 (1989).
Hayashida et al., Proc. Natl. Acad. Sci. USA, 87:9655-9659 (1990).
Lindqvist et al., Scandinavian J. Immunol., 38(3):267-272 (1993).
Luckow et al., Bio/Technology, 6:47-55 (1988).
Murata et al., J. Exp. Med., 175:341-351 (1992).
Tavenier et al., Cell, 66:1175-1184 (1991).
Thompsen et al., Bioprocess Technol., 17 (Insect Cell Culture Engineering):105-138 (1993).
Webb et al, Technique 2(4) 1990, pp. 173-188.
Gavernier et al, PNAS 89, 1992, pp. 7041-7045.
Leed; Current Biotechnology 1995, 6, pp. 567-573.

Affiliated with

Also associated with

Rating

Baculovirus expression system for human interleukin 5 receptor a does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Baculovirus expression system for human interleukin 5 receptor a, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Baculovirus expression system for human interleukin 5 receptor a will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1245957