Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1996-09-09
1998-08-11
Railey, III, Johnny F.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 711, 4351723, 4352523, 43525233, 4353201, 536 231, 536 232, 536 241, C12N 121, C12N 1511, C12N 2100, C12N 100
Patent
active
057926255
ABSTRACT:
Described herein is a bacterial cell containing a recombinant bacteriophage defense mechanism. The defense mechanism comprises a bacteriophage promoter (e.g., a phage .phi.31 promoter; a T7 promoter) operatively associated with a heterologous DNA encoding a product lethal to the bacterial cell. The bacterial cell is susceptible to infection by a bacteriophage, and the promoter is activated upon the infection of said bacterial cell by that bacteriophage. Bacteria useful in carrying out the invention include both gram negative and gram positive bacteria (e.g., Lactococcus lactis; Escherichia coli); the heterologous DNA may encode an enzyme that degrades nucleic acid (e.g., the products of the LlaI restriction cassette; barnase). Recombinant DNAs useful for making the foregoing cells, cultures prepared from such cells, and fermentation methods carried out with such cells are also disclosed.
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Gerdes et al., Proc. Natl. Acad. Sci. USA, 83:3116-3120, May 1986.
Yu et al., Proc. Natl. Acad. Sci. USA, 91:802-806, Jan. 1994.
Strittmatter et al., Biotechnology 13:1085-1089, Oct. 1995.
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Conkling Mark A.
Djordjevic Gordana
Klaenhammer Todd R.
O'Sullivan Dan
Taylor Christopher G.
North Carolina State University
Railey, III Johnny F.
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