Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Reexamination Certificate
2007-10-02
2007-10-02
Campell, Bruce R. (Department: 1648)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Reexamination Certificate
active
11438982
ABSTRACT:
The invention is a method for the development of a platform that enables the efficient and simultaneous creation of multiple detection assays. The invention entails the use of any bacteriophage, modified to carry a reporter gene, to which any analyte-recognizing moiety is attached via the capsid. The modified bacteriophage is used to attach to the target analyte. This attachment is identified via bacteriophage amplification in a helper bacteria strain with subsequent production and detection of the reporter gene.
REFERENCES:
Hazel, T.G., “Rapid, Ultra-Sensitive Detection ofE. coli0157:H7”, Scientific Conf. Abstract, Dept. of Marketing, Innovative Biosensors, Inc. 2005.
Petrenko, V.A. & Vodyanoy, V.J., “Phage Display for Detection of Biological Threat Agents”, Journal of Microbiological Methods, 53, 2003, 253-262.
Emanuel, P.A., Dang, J., Gebhardt, J.S., et al., “Recombinant Antibodies: A new Reagent for Biological Agent Detection”, Biosensors & Bioelectronics, 14, 2000, 751-759.
Gough, K.C., Cockburn, W., & Whitelam, G.C., “Selection of Phage-Display Peptides that Bind to Cucumber Mosaic Virus Coat Protein”, Journal of Virological Methods, 79, 1999, 169-180.
Rider, T.H., Petrovick, M.S., Nargi, F.E., et al. “A B Cell-Based Sensor for Rapid Identification of Pathogens”, Science, 301, Jul. 11, 2003, 213-215.
Campell Bruce R.
Harris Emily E.
Herink Kent A.
Kinsey Nicole E
University of Wyoming
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