Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1990-03-14
1991-01-22
Tieskin, Robin L.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 91, 4353201, 536 27, 935 8, 935 18, 935 77, 935 80, C12P 1934, C12N 1500, C07H 1512
Patent
active
049870734
ABSTRACT:
A bacteriophage directional cloning vector containing a multiple cloning site, a method for preparing cDNAs that can be cloned in a unique orientation and a bifunctional oligodeoxynucleotide linker, which creates a unique restriction site when ligated to the 3' end of cDNA fragments. The simple and efficient cDNA cloning system reduces the amount of RNA and effort required for the preparation of large directionally cloned libraries by providing a unique vector that accepts cDNA fragments in a unique 5'-3' orientation and by providing a unique bifunctional linker that facilitates the preparation of cDNA fragments that can be ligated directionally into the vector.
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Berman Michael L.
Meissner P. Scott
Sisk William P.
Akzo N.V.
Blackstone William M.
Bobrowicz Donna
Tieskin Robin L.
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