Bacteriolytic complex, method for producing said complex and...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

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C435S115000, C435S114000, C424S094100, C424S094600, C424S094200

Reexamination Certificate

active

07150985

ABSTRACT:
The inventions relate to the field of medicine, veterinary and biotechnology and may be used for production of a drug for medical and veterinary purposes. A bacteriolytic complex, produced by the bacteriumLysobactersp. XL1, has been proposed, which contains bacteriolytic enzymes (muramidase, muramoylalanineamidase, endopeptidase, a bacteriolytic enzyme with a molecular weight of about 22 kDa), protease, polysaccharide, and ballast components. The method of production of the bacteriolytic complex includes cultivation of the strain-producer on a nutrient medium containing glucose, peptone, yeast extract or yeast autolysate, phosphate salts of sodium and potassium, magnesium sulfate, potassium chloride, iron sulfate, and water.

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Stepnaya, O.A., et al “Lysoamidase: A Bacteriolytic Enzyme-Polysaccharide Complex of Microbial Origin . . . Polysaccharide”Biokhimiya, vol. 58, No. 10, p. 1523-1528, (1993).
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Stepnaya, O.A., et al. “Bacteriolytic enzymes”Uspekhi biologicheskoy khimii, vol. XXXIX, p. 341 (1999).
Likhosherstov, L.M., et al. “Structure of an acidic polysaccharide present in the bacteriolytic complex lysoamidase”FEBS Letters, vol. 368, p. 113-116, (1995).

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