Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1994-02-09
1996-03-19
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 693, 435 697, 435177, 435209, 4352523, 514 6, 530350, 530395, 530400, 536 221, 536 231, 536 234, 536 237, 42419711, 4241921, C12P 2106, C07K 100, A61K 3800, C07H 1900
Patent
active
055003532
ABSTRACT:
This invention provides a bacterium having an S-layer modified such that the bacterium S-layer protein gene contains one or more in-frame sequences coding for one or more heterologous polypeptides and, the S-layer is a fusion product of the S-layer protein and the heterologous polypeptide. The bacterium is preferably a Caulobacter which may be cultured as a film in a bioreactor or may be used to present an antigenic epitope to the environment of the bacterium. This invention also provides a method of expressing and presenting to the environment of a Caulobacter, a polypeptide that is heterologous to the S-layer of Caulobacter which comprises cloning a coding sequence for the polypeptide in-frame into an S-layer protein gene of Caulobacter whereby the polypeptide is expressed and presented on the surface of the Caulobacter as a fusion product of the S-layer protein and the polypeptide in the S-layer of the Caulobacter.
REFERENCES:
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Fisher, James A.; John Smit; and, Nina Agabian; "Transcriptional Analysis of the Major Surface Array Gene of Caulobacter crescentus" (1988) J. Bacteriol 170, 4706-4713.
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Hayes, L. J.; et al; "Chlamydia trachomatis Major Outer Membrane Protein Epitopes Expressed as Fusions with LamB in an Attenuated aroA Strain of Salmonella typhimuirum; Their Application as Potential Immunogens" (1991) J. General Microbiology 137, 1557-1564.
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Bingle, W. H., et al, "Definition of Form and Function for the S-Layer of Caulobacter crescentus" in: Advances in Bacterial Paracrystalline Surface Layers (1993) Plenum Press (T. J. Beveridge and S. F. Koval ed.), pp. 181-197 (see enclosed author's copy insert at pp. 9-11).
Bingle, W. H., et al, "Linker Mutagenesis of the Caulobacter crescentus S-Layer Protein", in: Advances in Bacterial Paracrystalline Surface Layers (1993) Plenum Press (T. J. Beveridge and S. F. Koval ed.), pp. 293-296.
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Bingle Wade H.
Smit John
Kim Hyosuk
The University of British Columbia
Wax Robert A.
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