Bacterial strains of the genus Bacillus, closely phenotypically

Details Bacterial strains of the genus Bacillus, closely phenotypically Bacterial strains of the genus Bacillus, closely phenotypically

1996-01-10

2000-02-08

Caputa, Anthony C.

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

435 691, 4352521, 435853, 435854, 435855, 435856, 435857, A01N 6300, C12P 2106, C12N 112, C12N 100

Patent

active

060225378

DESCRIPTION:

BRIEF SUMMARY
BACKGROUND OF THE INVENTION

1. Field of the Invention
The invention relates to bacterial strains closely phenotypically related to the genus Lactobacillus.
It also relates to methods for culture of these strains and to their industrial uses.
The invention more particularly relates to bacterial strains isolated from palm wine.
2. Description of Related Art
It is known that palm wine is an alcoholic drink originating from spontaneous fermentation of the sap of trees belonging to the palm tree family. This sap comprises about 10 to 15% (w/v) of sucrose, several amino acids and vitamins.


BRIEF DESCRIPTION OF THE DRAWINGS.

FIG. 1 shows a photograph of cells of the invention under an optical microscope.
FIG. 2 shows a photograph of cells of the invention under an electron microscope with a magnification of 20,000.
FIG. 3 shows a photograph of cells of the invention under an electron microscope with a magnification of 80,000.


DETAILED DESCRIPTION OF THE INVENTION

Microbiological studies which have been carried out have demonstrated the presence of an abundant mesophilic flora made up of several species of bacteria and yeasts.
A study of the thermophilic anaerobic flora present in a palm wine originating from Senegal by the inventors led them to isolate a strain which is still unidentified and has properties of great advantage in various fields of industry.
The object of the invention is thus to provide new strains of this type.
It also relates to the provision of culture protocols for these strains specifying the physico-chemical conditions and the composition of the culture medium which allow favourable production of cells and/or certain metabolites, such as, for example, L(+)-lactate.
According to another aspect, the invention relates to the direct use of these strains or that of their metabolites in various industries.
The bacterial strains of the invention are characterized in that they have a DNA sequence of which at least part is capable of hybridizing with at least part of the genome or plasmid DNA of the strain deposited at the Collection Nationale de Cultures de Microorganismes (C.N.C.M.=National Collection of Microorganism Cultures) on Nov. 24, 1993 under no. I-1378.
Advantageously, at least 70% of the genome of the strains of the invention is capable of hybridizing with the DNA of the strain deposited.
According to another aspect, these strains are characterized in that they are moderately thermophilic, have amylolytic properties and/or are capable of producing lactate from carbohydrates. It is pointed out that the lactate produced is advantageously L(+)-lactate to the extent of more than 95%.
The expression "moderately thermophilic" is understood as meaning bacterial strains which are capable of growing at temperatures of the order of 25 to 58.5.degree. C., with an optimum between 47 and 53.degree. C.
The invention more particularly relates to strains of the genus Bacillus as demonstrated by comparison of RNA sequences of the 16 S fraction of the ribosomes.
Strains of this type are also characterized in that they are heterolactic, that is to say they are capable of producing compounds other than L(+)-lactate, in particular ethanol, formate and acetate, from carbohydrates, depending on the physico-chemical conditions of the culture medium, in particular depending on the pH and the sugars/peptides ratio.
Such strains are also characterized in that they have no cytochrome and are not capable of utilizing nitrates or sulphates as a terminal electron acceptor.
They have a catalase and their metabolism is facultatively anaerobic.
According to another embodiment of the invention, these strains are characterized by anaerobic growth conditions and microaerophilic properties, that is to say low partial pressures of oxygen stimulate their growth.
Their growth takes place more specifically at a pH of 5.4 to 8.5, with an optimum for growth of about 6.8 to 7.2.
The bacterial strains of the invention are also characterized in that they are Gram-positive.
According to another embodiment of the invention, the b

REFERENCES:
Combet-Blanc et al. International Journal of Systematic Bacteriology 45(1):9-16 (see abstract), Jan. 1995.
Delaporte et al. C.R. Hebd. Seances Acad. Sci., Ser. D. 264(19):2344-6 (see abstract), 1967.
Burgess et al J. Cell Biology 111: 2129-2137, Apr. 1990.
Lazar et al. Mol. Cell Biology 8(3): 1247-52, Mar. 1988.
Wayne et al (Journal of Systematic Bacteriology, vol. 37, No. 4, Oct. 1987, p. 463-464).

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