Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-08-01
2006-08-01
Navarro, Mark (Department: 1645)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S007100, C435S007200, C435S007320, C435S007400
Reexamination Certificate
active
07083918
ABSTRACT:
A transgenic bacterial cell comprising(a) a dimeric small molecule which comprises a first moiety known to bind a first receptor domain covalently linked to a second moiety known to bind a second receptor domain;(b) nucleotide sequences which upon transcription encodei) a first fusion protein comprising the first receptor domain, andii) a second fusion protein comprising the second receptor domain; and(c) a reporter gene wherein expression of the reporter gene is conditioned on the proximity of the first fusion protein to the second fusion protein. The cell is also adapted for use in a method for identifying a molecule that binds to a known target in a bacterial cell from a pool of candidate molecules, and a method for identifying an unknown target receptor to which a molecule is capable of binding in a bacterial cell. Also described are compounds and kits for carrying out the methods.
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Althoff Eric A.
Cornish Virginia W.
Cooper & Dunham LLP
Navarro Mark
The Trustees of Columbia University in the City of New York
White, Esq. John P.
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