Bacterial shuttle vectors

Details Bacterial shuttle vectors Bacterial shuttle vectors

1994-01-21

1996-09-24

LeGuyader, John L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 697, 435 698, 4351723, 4352523, 43525231, 43525233, 435853, 536 231, 536 234, 536 237, 536 241, C12N 1570, C12N 1574

Patent

active

055590071

ABSTRACT:
The invention relates to the field of genetic engineering and provides novel DNA molecules which comprise multifunctional origins of replication and/or a DNA sequence coding for the most abundant protein in the supernatant of cultures of Lactococcus Spec., named Major Secretion Product (MSP), for its signal peptide and/or the promoter of the MSP gene. The novel DNA molecules are used for the production of novel shuttle vectors for cloning of DNA in at least E. coli and Lactococcus spec. or novel vectors for the expression of homologous or heterologous genes and the production of secreted gene products in gram positive bacteria such as Lactococcus spec. and Bacillus Spec.

REFERENCES:
Maniatis et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 1982, pp. 429, 430 & 433 (422 & 249 previously provided).
Maniatis et al. Molecular Cloning, CSH 1982 pp. 3-4, 249 & 422.
Chassy, FEMS Microbiology Reviews, vol. 46, pp. 297-312 (1987).
Gasson et al., FEMS Microbiology Reviews, vol. 30, pp. 193-196 (1985).
De Vos, FEMS Microbiology Reviews, vol. 46, pp. 281-295 (1987).
Jos et al., Applied and Environmental Microbiology, vol. 50, pp. 540-542 (1985).
Harlander et al., Applied and Environmental Microbiology, vol. 48, pp. 347-351 (1984).
Davies et al., J. Appl. Bacteriol., vol. 51, pp. 325-337 (1981).
Aschen et al., Gene, vol. 45, pp. 45-49 (1986).
Gasson, J. Bacteriol., vol. 154, pp. 1-9 (1983).
Macrina et al., Gene, vol. 19, pp. 345-353 (1982).
Simmons et al., J. Dairy Sci.,71:83 (1988), Abstract D64.
De Vos et al., J. Dairy Sci., 72:3398-3405 (1989).
De Vos, Netherlands Milk and Dairy Journal, 40:141-154 (1986).
van Asseldonk et al., Gene, 95:155-160 (1990).

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