Bacterial production of heterologous polypeptides under the cont

Details Bacterial production of heterologous polypeptides under the cont Bacterial production of heterologous polypeptides under the cont

1982-05-05

1985-02-12

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

435 68, 4351723, 935 41, C12P 2102, C12P 2100, C12N 1500

Patent

active

044991886

ABSTRACT:
A process for bacterially producing heterologous polypeptides, particularly those such as human IFN-.beta. that inhibit bacterial growth, in which bacteria that have been transformed to express the heterologous polypeptide under the control of a trp promoter-operator are cultivated in a known volume of medium containing an excess of a preferred carbon source such as glucose and a predetermined amount of tryptophan that corresponds approximately to the amount of tryptophan contained in the bacteria in the volume of medium at a predetermined elevated cellular density, whereby expression of the heterologous polypeptide is substantially repressed until the bacteria grow to approximately the predetermined elevated cellular density and is thereafter automatically derepressed to permit expression of the heterologous polypeptide.

REFERENCES:
patent: 4374927 (1983-02-01), Sninsky et al.
Mandelstam et al., Biochemistry of Bacterial Growth, John Wiley & Sons, pp. 159 & 160, (1982).
Derynck et al., Nature, vol. 285, pp. 542-546, Jun. 1980.
Taniguchi et al., Nature, vol. 285, pp. 546-549, Jun. 1980.
Edge et al., Nature, vol. 292, pp. 756-762, Aug. 1981.
Crawford et al., Ann. Rev. Biochem., vol. 49, pp. 163-195 (1980).

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