Bacterial polypeptide expression employing tryptophan promoter-o

Details Bacterial polypeptide expression employing tryptophan promoter-o Bacterial polypeptide expression employing tryptophan promoter-o

1993-04-29

1999-03-30

Scheiner, Laurie

Chemistry: molecular biology and microbiology

Vector, per se

435 691, 435 694, 43525233, 435471, 435476, 435488, 536 2351, C12N 1563, C12N 1570

Patent

active

058888080

ABSTRACT:
The present invention provides recombinant DNA vehicles which are suitable for the microbial expression of DNA encoding a heterologous polypeptide which comprises a portion of the trp operon having the promoter-operator and leader ribosome binding site, and a restriction site providing an insertion site for the DNA sequences encoding the heterologous polypeptide, wherein the restriction site is located 3' of the leader ribosome binding site as a substitute for the Taq I site of the trp promoter-operator and is selected from the group consisting of Xba I and Eco RI. Also provided are E. coli strains transformed with the above described recombinant DNA vehicles.

REFERENCES:
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patent: 5145782 (1992-09-01), Hallewell
Jeffrey H. Miller, Experiments in Molecular Genetics, "Construction of trpR.sup.- Derivatives of trp-lac Fusion Strains", pp. 312-315, Cold Spring Harbor Laboratory, 1972.
Miozzari, et al., J. Bacteriol., 133(3): 1457-1466 (1978).
Rodriguez, et al., Nucleic Acids Research, 6(10): 3267-3287 (1979).
Goeddel, et al., Nature, 281: 544-548 (1979).
Miozzari, et al., J. Bacteriol., 134(1):48-59 (1978).
Goeddel et al, Proc. Natl. Acad. Sci. USA, vol. 76, No. 1, Jan. 1979, "Expression in Escherichia coli of Chemically Synthesized Genes for Human Insulin", pp. 106-110.

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