Bacterial methionine n-terminal peptidase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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935 12, 935 14, 935 78, 536 27, 435 6, 435 691, 435 712, 4351721, 4351723, 4352523, 4353201, C12N 948, C12N 1510, C12N 1557

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050136623

ABSTRACT:
Method of obtaining N-terminal methionine-free proteins are described. The methods employ a novel enzyme, E. coli methionine aminopeptidase either in vitro or in vivo. For in vivo application, plasmid-borne DNA encoding the peptidase is transformed into a bacterial host which produces the desired protein.

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