Bacterial methionine N-terminal peptidase

Details Bacterial methionine N-terminal peptidase Bacterial methionine N-terminal peptidase

1985-09-20

1989-09-26

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 68, 4351723, 43525233, 4353171, C12N 948, C12N 1500

Patent

active

048700177

ABSTRACT:
Methods of obtaining N-terminal methionine-free proteins are described. The methods employ a novel enzyme, E. coli methionine aminopeptidase either in vitro or in vivo. For in vivo application, plasmid-borne DNA encoding the peptidase is transformed into a bacterial host which produces the desired protein.

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