Bacterial isolates of the genus Klebiella , and an...

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Nonplant protein is expressed from the polynucleotide

Reexamination Certificate

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C800S278000, C800S284000, C800S300100, C800S308000, C536S023100, C536S023200, C536S023700, C536S023400, C435S069100, C435S468000, C435S320100, C435S412000, C435S069800

Reexamination Certificate

active

10203963

ABSTRACT:
Two strains of a novel bacterial species,Klebsiella singaporensisLX3 and LX21, are claimed. A nucleotide sequence (kis) encoding a novel form of isomaltulose synthase, KIS, is also claimed. Also claimed is a method for production of isomaltulose in a plant, which method comprises introducing into a cell of such plant a nucleic acid sequence which encodes an enzyme which converts sucrose into isomaltulose in a manner which allows said cell to express said nucleic acid sequence, as well as a functional cloning method of isolating nucleotide sequence encoding the KIS protein comprising the steps of (a) preparing a gene bank from a donor organism that contains a DNA sequence coding for an isomaltulose biosynthesis activity in a suitable host organism, (b) screening the clones of interest from the gene bank by their enhanced reducing sugar content, and (c) isolating the clones which contain a DNA coding for a protein with isomaltulose biosynthesis activity.

REFERENCES:
patent: 5786140 (1998-07-01), Mattes et al.
patent: 5866791 (1999-02-01), Holt
patent: 5985668 (1999-11-01), Klein et al.
patent: WO 95/20047 (1995-07-01), None
Zhang et al 2002 Applied and Environmental Microbiology 68:2676-2682 p. 2676.
Stephanopoulos et al 1993 Trends in Biotechnology 11:392-396 pp. 393 and 394.
Tsuyuki, et al., “Isolation and characterization of isomaltulose- and trehalulose-producing bacteria from Thailand soil.”Journal of General and Applied Microbiology, vol. 38, No. 5, 1992, pp. 483-490.
Huang, et al., “Conversion of sucrose to isomaltulose byKlebisella planticolaCCRC 191112.”Journal of Industrial Microbiology and Biotechnology, vol. 21, No. 1-2, Jul. 1998, pp. 22-27.
Park, et al., “Conversion of sucrose to isomaltulose by microbial glucosyltransferase.”Biotechnology Letters, vol. 14, No. 7, 1992, pp. 547-551.

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