Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Nonplant protein is expressed from the polynucleotide
Reexamination Certificate
2007-06-26
2007-06-26
Fox, David T. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Nonplant protein is expressed from the polynucleotide
C800S278000, C800S284000, C800S300100, C800S308000, C536S023100, C536S023200, C536S023700, C536S023400, C435S069100, C435S468000, C435S320100, C435S412000, C435S069800
Reexamination Certificate
active
10203963
ABSTRACT:
Two strains of a novel bacterial species,Klebsiella singaporensisLX3 and LX21, are claimed. A nucleotide sequence (kis) encoding a novel form of isomaltulose synthase, KIS, is also claimed. Also claimed is a method for production of isomaltulose in a plant, which method comprises introducing into a cell of such plant a nucleic acid sequence which encodes an enzyme which converts sucrose into isomaltulose in a manner which allows said cell to express said nucleic acid sequence, as well as a functional cloning method of isolating nucleotide sequence encoding the KIS protein comprising the steps of (a) preparing a gene bank from a donor organism that contains a DNA sequence coding for an isomaltulose biosynthesis activity in a suitable host organism, (b) screening the clones of interest from the gene bank by their enhanced reducing sugar content, and (c) isolating the clones which contain a DNA coding for a protein with isomaltulose biosynthesis activity.
REFERENCES:
patent: 5786140 (1998-07-01), Mattes et al.
patent: 5866791 (1999-02-01), Holt
patent: 5985668 (1999-11-01), Klein et al.
patent: WO 95/20047 (1995-07-01), None
Zhang et al 2002 Applied and Environmental Microbiology 68:2676-2682 p. 2676.
Stephanopoulos et al 1993 Trends in Biotechnology 11:392-396 pp. 393 and 394.
Tsuyuki, et al., “Isolation and characterization of isomaltulose- and trehalulose-producing bacteria from Thailand soil.”Journal of General and Applied Microbiology, vol. 38, No. 5, 1992, pp. 483-490.
Huang, et al., “Conversion of sucrose to isomaltulose byKlebisella planticolaCCRC 191112.”Journal of Industrial Microbiology and Biotechnology, vol. 21, No. 1-2, Jul. 1998, pp. 22-27.
Park, et al., “Conversion of sucrose to isomaltulose by microbial glucosyltransferase.”Biotechnology Letters, vol. 14, No. 7, 1992, pp. 547-551.
Li Xianzhen
Zhang Daohai
Zhang Lian Hui
Agency for Science Technology and Research
Fox David T.
Page Brent T
Rothwell Figg Ernst & Manbeck P.C.
LandOfFree
Bacterial isolates of the genus Klebiella , and an... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Bacterial isolates of the genus Klebiella , and an..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Bacterial isolates of the genus Klebiella , and an... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3855923