Bacterial host strains for producing proteolytically sensitive p

Details Bacterial host strains for producing proteolytically sensitive p Bacterial host strains for producing proteolytically sensitive p

1993-11-18

1996-04-16

Fleisher, Mindy B.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 691, 4351723, C12N 120, C12N 1501

Patent

active

055081924

ABSTRACT:
The invention relates to methods of producing recombinant polypeptides in protease-deficient bacterial hosts. Constructs of single, double, triple and quadruple protease deficient and protease/rpoH mutants of E. coli are described. Proteolytically sensitive polypeptides may be expressed and secreted in such cells, providing significantly increased yields compared with expression in wild-type strains.

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