Bacterial host cell for the direct expression of peptides

Details Bacterial host cell for the direct expression of peptides Bacterial host cell for the direct expression of peptides

2007-10-30

2009-06-30

Ton, Thaian N. (Department: 1632)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S252100, C435S320100

Reexamination Certificate

active

07553655

ABSTRACT:
Expression systems are disclosed for the direct expression of peptide products into the culture media where genetically engineered host cells are grown. High yield was achieved with a special selection of hosts, and/or fermentation processes which include careful control of cell growth rate, and use of an inducer during growth phase. Special universal cloning vectors are provided for the preparation of expression vectors which include control regions having multiple promoters linked operably with coding regions encoding a signal peptide upstream from a coding region encoding the peptide of interest. Multiple transcription cassettes are also used to increase yield. The production of amidated peptides using the expression systems is also disclosed.

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