Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2005-03-08
2005-03-08
Smith, Lynette R. F. (Department: 1645)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S023100, C536S023400, C424S184100, C424S192100, C424S199100, C530S501000
Reexamination Certificate
active
06864365
ABSTRACT:
The invention provides a system for creating recombinant agfA fimbrin genes and performing chromosomal gene replacements withinSalmonella, creatingSalmonellastrains which carry the recombinant agfA genes at the native position in the chromosome. One embodiment of the invention is exemplified by the expression of a model epitope (PT3) obtained from the GP63 protein ofLeishmania major, by formation of recombinant agfA genes encoding PT3 fusion proteins recombined at 10 different sites throughout the agfA gene. These fusions are shown to be expressed in the thin aggregative fimbriae on the surface of bacterial cell. The agfA fimbrin ofSalmonella(CsgA forE. coli) provides a flexible and stable vehicle for the expression of foreign epitopes in enterobacteriaceae and the subsequent thin aggregative fimbriae (curli) expression product provide an ideal organelle for presentation of the foreign epitopes at the cell surface.
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Eshdat et al.,
Collinson S. Karen
Doran James L.
Kay William W.
White Aaron P.
Ford Vanessa L.
Innovation and Development Corporation, University of Victoria
Seed IP Law Group PLLC
Smith Lynette R. F.
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