Bacterial expression of porcine growth hormone

Details Bacterial expression of porcine growth hormone Bacterial expression of porcine growth hormone

1992-02-24

1993-10-26

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

43525233, 4353201, C12P 2102, C12N 1518, C12N 1573, C12N 1503

Patent

active

052565467

ABSTRACT:
A plasmid for the production of porcine growth hormone or an analog thereof which upon introduction into a suitable bacterial host containing the thermolabile repressor C.sub.I renders the host cell capable, upon increasing the temperature of the host cell to a temperature at which the repressor is destroyed, of effecting expression of DNA encoding porcine growth hormone and production of porcine growth hormone or analogs thereof. The plasmid is a double-stranded DNA molecule which includes in 5' to 3' the following: a DNA sequence which contains the promoter and operator PLOL from lambda bacteriophage; the N utilization site for binding antiterminator N protein produced by the host cell; a DNA sequence which contains a ribosomal binding site for rendering the mRNA of the gene encoding porcine growth hormone capable of binding to ribosomes within the host cell; an ATG initiation codon; a restriction enzyme site for inserting the gene encoding porcine growth hormone into the plasmid in phase with the ATG initiation codon; a gene encoding porcine growth hormone; and additionally a DNA sequence which contains an origin of replication from a bacterial plasmid capable of autonomous replication in the host cell and a DNA sequence which contains a gene associated with a selectable or identifiable trait which is manifested when the plasmid is present in the host cell.

REFERENCES:
patent: 4578355 (1986-03-01), Rosenberg
patent: 5126252 (1992-06-01), Oppenheim et al.
Rosenberg et al; Methods in Enzymology 101: 123 (1983).
Seeberg et al; DNA 2: 37 (1983).
Movva et al; Chem. Abstr. 101: 18489e (1984).

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