Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-01-17
1998-06-09
Degen, Nancy
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 712, 43525231, 4352525, 4353201, 536 237, C12Q 168, C12P 2100, C12N 1565, C07H 2104
Patent
active
057631875
ABSTRACT:
The present invention relates to a modified bacterial donor cell useful in conjugation, the cell containing i) a plasmid comprising a DNA construct encoding the polypeptide of interest, and at least one cis-acting DNA sequence required for the transfer of said plasmid by conjugation in the presence of a trans-acting mobilizing element, and ii) at least one DNA sequence encoding said trans-acting mobilizing element, wherein the donor cell has a reduced capacity, relative to a parent cell, of producing a bactericidal agent which kills or prevents growth in a population of recipient cells.
REFERENCES:
patent: 5346818 (1994-09-01), Schafer et al.
Slepecky et al. The genus Bacillus-nonmedical. in: The prokaryotes, 2nd ed., Eds. Balows, Truper, Dworkin, Harder and Schleifer, Springer-Verlag. vol. 2:1663-1693, Dec. 1991.
Hendrick et al. Insertino of Tn916 into Bacillus pumilus plasmid pMGD302 adn evidence for plasmid transfer by conjugation. Plasmid vol. 26:1-9, Sep. 11, 1991.
Jacobs et al. Bacillus subtilis PrsA is required in vivo as an extracytoplasmec chaperone for secretin of active enzymes synthesized either with or without pro-sequences. Mol. Microbiol. vol. 8(5):957-966, May 1993.
Selinger et al. Mobilization of closely related plasmids pUB110 and pBC16 by Bacillus plasmid pXO503 requires trans-acting open reading frame beta. J. Bact. vol. 172(6):3290-3297, Jun. 1990.
Sloma Alan P.
Widner William R.
Degen Nancy
Lambiris, Esq. Elias J.
Novo Nordisk Biotech Inc.
Sandals William
Zelson Esq. Steve T.
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