Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Reexamination Certificate
2006-05-16
2006-05-16
Sullivan, Daniel M. (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
C435S245000, C435S252300, C435S091500
Reexamination Certificate
active
07045336
ABSTRACT:
We describe a bacterial delivery system for the delivery of DNA and antigens into cells. We constructed an attenuated bacterial vector which enters mammalian cells and ruptures delivering functional plasmid DNA and antigens into the cell cytoplasm. ThisShigellavector was designed to deliver DNA to colonic surfaces, thus opening the possibility of oral and other mucosal DNA immunization and gene therapy strategies. The attenuatedShigellais also useful as a vaccine for reducing disease symptoms caused by Shigella.
REFERENCES:
patent: 4888170 (1989-12-01), Curtiss
patent: 5112749 (1992-05-01), Brey et al.
patent: 5468485 (1995-11-01), Curtiss
patent: 5672345 (1997-09-01), Curtiss, III
patent: 5824538 (1998-10-01), Branstrom et al.
patent: 5877159 (1999-03-01), Powell et al.
Sizemore et al., Vaccine 15(8):804-807 (1997).
Van De Verg et al. (1995) Antibody and cytokine responses in a mouse pulmonary model ofShigellaflexneriserotype 2a infection.Infec. Immun.63:1947-1954.
Sun et al. (1994) Cholera toxin B subunit: an efficient transmucosal carrier-delivery system for induction of peripheral immunological tolerance.PNAS91: 10795-10799.
ASM Meeting News, 95th General Meeting, Washington, D.C. May 23, 1995. Mucosal surfaces present a new vaccine approach.
Zychlinsky et al. (1992)Shigella flexneriinduces apoptosis in infected macrophages.Nature358:167-169.
Hartman et al. (1991) Small-animal model to measure efficacy and immunogenicity ofShigellavaccine strains.Infec. Immun.59: 4075-4083.
Oaks et al. (1985) Plaque formation by virulentShigella flexneri. Infec. Immun.48: 124-129.
Mills et al. (1998)Shigella flexneriinvasion plasmid antigens B and C: epitope location and characterization with monoclonal antibodies.Infec. Immun.56:2933-2941.
Hartman et al. (1944) Local immune response and protection in the guinea pig keratoconjunctivitis model following immunization withShigellavaccines.Infec. Immun62: 412-420.
Donnelly et al. (1994) Immunization with DNA.J. Immun. Methods176: 145-152.
Nakayama et al. (1988) Construction of an ASD+expression-cloning vector: stable mainternance and high level expression of cloned genes in aSalmonellavaccine strain.Bio/Technology6: 693-697.
Sansonetti et al. (1983) Alterations in the pathogenicity ofEscherichia coliK12 after transfer of plasmid and chromosomal genes fromShigella flexneri.. Infec. Immun.39: 1392-1402.
Sizemore, D. R. et al. (1995) AttenuatedShigellaas a DNA delivery vehicle for DNA-mediated immunization.Science270:299-302.
Branstrom Arthur A.
Sadoff Jerald C.
Sizemore Donata R.
Arwine Elizabeth
Harris Charles H.
Moran John Francis
Sullivan Daniel M.
The United States of America as represented by the Secretary of
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