Bacterial catabolism of chitin

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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435200, 435209, 4353201, 435909, 536 232, 536 237, C12N 120, C12N 924, C12N 100, C07H 2104

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059856440

ABSTRACT:
Three genes involved in the catabolism of chitin in Vibrio furnissii: endI encodes periplasmic chitodextrinase, exoI encodes periplasmic .beta.-N-acetylglucosaminidase, and exoII encodes aryl .beta.-N-acetylglucosaminidase are provided. The complete nucleotide sequence for each of the three genes and the complete amino acid for the corresponding enzymes are demonstrated along with host cells capable of expressing the recombinant enzymes. The present invention also describes four specific strains of V. furnissii having deletions in genes involved in the catabolic pathway of chitin and a process for the production of chitin oligosaccharides.

REFERENCES:
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B. Bassler, P. Gibbons, C. Yu, and S. Roseman; (Chitin Utilization by Marine Bacteria); Oct. 18, 1990; The Journal of Biological Chemistry; vol. 266, No. 36, Issue of Dec. 25, pp. 24268-24275, 1991.
B. Bassler, C. Yu, Y.C. Lee, and S. Roseman; (Chitin Utilization by Marine Bacteria); Oct. 18, 1990; The Journal of Biological Chemistry; vol. 266, No. 36, Issue of Dec. 25, pp. 24276-24286, 1991.
C. Yu, B. Bassler, and S. Roseman: (Chemotaxis of the Marine Bacterium Vibrio Furnissii to Sugars); The Journal of Biological Chemistry; vol. 268, No. 13, Issue May 5, pp. 9405-9409, 1993.
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Tsujibo, et al., Molecular Cloning of the Gene Which Encodes .beta.-N-Acetylglucosaminidase from a Marine Bacterium, Alteromonas sp. Strain 0-7, Applied and Environmental Microbiology, 61(2):804, Feb. 1995.
Yamano, et al., Production of N-Acetylglucosamine Deacetylase by Vibrio cholerai Non-01, Biosci. Biotech Biochem., 58(1):193, 1994.
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