Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2000-11-30
2008-11-04
Chen, Stacy B (Department: 1648)
Chemistry: molecular biology and microbiology
Vector, per se
C424S221100
Reexamination Certificate
active
07445928
ABSTRACT:
The present invention relates to methods of preparing a DNA comprising steps, wherein (a) a DNA comprising a full length copy of the genomic RNA (gRNA) or an RNA virus; or (b) a DNA comprising one or several fragments of a gRNA of an RNA virus, which fragments code for an RNA dependent RNA polymerase and at least one structural or non-structural protein; or (c) a DNA having a homology of at least 60% to the sequences of (a) or (b); is cloned into a bacterial artificial chromosome (BAC). Additionally, DNAs are provided, which comprise sequences derived from the genomic RNA (gRNA) of a coronavirus which sequences have a homology of at least 60% to the natural sequence of the virus and code for an RNA dependent RNA polymerase and at least one structural or no-structural protein, wherein a fragment of said DNA is capable of being transcribed into RNA which RNA can be assembled to a virion. Further, the use of these nucleic acids for preparation of viral RNA or virions as well as pharmaceutical preparations comprising these DNAs, viral RNAs or virions is disclosed.
REFERENCES:
patent: 5288641 (1994-02-01), Roizman
patent: 5501979 (1996-03-01), Geller et al.
patent: 5585096 (1996-12-01), Martuza et al.
patent: 5658724 (1997-08-01), DeLuca
patent: 5776745 (1998-07-01), Ketner et al.
patent: 6277621 (2001-08-01), Horsburgh et al.
patent: 6593111 (2003-07-01), Baric et al.
patent: 0 453 242 (1991-10-01), None
patent: WO 90/09441 (1990-08-01), None
patent: WO 95/03400 (1995-02-01), None
patent: WO 96/04394 (1996-02-01), None
patent: WO 96/15779 (1996-05-01), None
patent: WO 96/26267 (1996-08-01), None
patent: WO 97/05263 (1997-02-01), None
patent: WO 97/30732 (1997-08-01), None
patent: WO 97/34008 (1997-09-01), None
patent: WO 99/06582 (1999-02-01), None
patent: WO 99/43842 (1999-09-01), None
patent: WO 01/39797 (2001-06-01), None
P. Ahlquist et al., “Multicomponent RNA Plant Virus Infection Derived From Cloned Viral cDNA”, Proc. Natl. Acad. Sci. USA, Nov. 1984, pp. 7066-7070, vol. 81, The National Academy of Sciences, Washington, DC.
F. Almazan et al., “Engineering the Largest RNA Virus Genome as an Infectious Bacterial Artificial Chromosome”, Proc. Natl., Acad. Sci. USA, May 2000, pp. 5516-5521, vol. 97, No. 10, The National Academy of Sciences, Washington, DC.
F. Ascenzioni et al., “Mammalian Artificial Chromosomes-Vectors for Somatic Gene Therapy”, Cancer Letters, 1997, pp. 135-142, vol. 118, Elsevier Science Ireland Ltd., Ireland.
M.L. Ballesteros et al., “Two Amino Acid Changes at the N-Terminus of Transmissible Gastroenteritis Coronavirus Spoke Protein Result in the Loss of Enteric Tropsim”, Virology, 1997, pp. 378-388, vol. 227, Academic Press, Inc., San Diego, CA.
M.D. Baron et al., “Rescue of Rinderpest Virus From Cloned cDNA”, J. Virol., Feb. 1997, pp. 1265-1271, vol. 71, No. 2, American Society for Microbiology, Washington, DC.
G. Bilbao et al., “Adenoviral/Retroviral Vector Chimeras: A Novel Strategy to Achieve High-Efficiency Stable Transduction in vivo”, The FASEB Journal, Jul. 1997, pp. 624-634, vol. 11, FASEB, Bethesda, MD.
J.-C. Boyer et al., “Infectious Transcripts and cDNA Clones of RNA Viruses”, Virology, 1994, pp. 415-426, vol. 198, Academic Press, Inc., San Diego, CA.
Burke, “Special Section: Yeast Artificial Chromosome Cloning; YAC Cloning: Options and Problems”, Genet. Anal. Tech. Appl., 1990, pp. 94-99, vol. 7, No. 5, Elsevier Science Publishing Co., Inc., NY, NY.
R. Casais et al., “Reverse Genetics System for the Avian Coronavirus Infectious Bronchitis Virus”, J. Virol., Dec. 2001, pp. 12359-12369, vol. 75, No. 24, ASM Press, Washington, DC.
R.-Y. Chang et al., “Acis-Acting Function for the Coronavirus Leader in Defective Interfering RNA Replication”, J. Virol., Dec. 1994, pp. 8223-8231, vol. 68, No. 12, American Society for Microbiology, Washington, DC.
H.C. Chiou et al., “Mutations in the Herpes Simplex Virus DNA-Binding Protein Gene Leading to Altered Sensitivity to DNA Polymerase Inhibitors”, Virology, 1985, pp. 213-226, vol. 145, Academic Press, Inc., San Diego, CA.
P. L. Collins et al., “Production of Infectious Human Respiratory Syncytial Virus From Cloned cDNA Confirms an Essential Role for the Transcription Elongation Factor From the 5′ Proximal Open Reading Frame of the M2 mRNA in Gene Expression and Provides a Capability for Vaccine Development”, Proc. Natl. Acad. Sci. USA, Dec. 1995, pp. 11563-11567, vol. 92, The National Academy of Sciences, Washington, DC.
N.L. Davis et al., “In vitro Synthesis of Infectious Venezuelan Equine Encephalitis Virus RNA From a cDNA Clone: Analysis of a Viable Deletion Mutant”, Virology, 1989, pp. 189-204, vol. 171, Academic Press, Inc., San Diego, CA.
T.W. Dubensky, Jr. et al., “Sindbis Virus DNA-Based Expression Vectors : Utility For in vitro and in vivo Gene Transfer”, J. Virol., Jan. 1996, pp. 508-519, vol. 70, No. 1, American Society for Microbiology, Washington, DC.
A.P. Durbin et al., “Recovery of Infectious Human Parainfluenza Virus Type 3 From cDNA”, Virology, 1997, pp. 323-332, vol. 235, Academic Press, Inc., San Diego, CA.
L. Enjuanes et al., “Coronaviruses andArteriviruses”, 1998, Plenum Press, New York.
L. Enjuanes et al., “Molecular Basis of Transmissible Gastroenteritis Virus Epidemiology”,InTheCoronaviridae, 1995, S.G. Siddell (Ed.) Plenum Press, New York, pp. 337-376.
I. Frolov et al., “Alphavirus-Based Expression Vectors: Strategies and Applications”, Proc. Natl. Acad. Sci. USA, Oct. 1996, pp. 11371-11377, vol. 93, The National Academy of Sciences, Washington, DC.
P.J. Gage et al., “A cell-Free Recombination System for Site-Specific Integration of Multigenic Shuttle Plasmids Into the Herpes Simplex Virus Type 1 Genome”, J. Virol., Sep. 1992, pp. 5509-5515, vol. 66, No. 9, American Society for Microbiology, Washington, DC.
D. Garcin et al., “A highly Recombinogenic System for the Recovery of Infectious Sendai Paramyxovirus From cDNA: Generation of a Novel Copy-Back Nondefective Interfering Virus”, EMBO J., 1995, pp. 6087-6094, vol. 14, No. 24, Oxford University Press, Oxford, UK.
U. Geigenmüller et al., “Construction of a Genome-Length cDNA Clone for Human Astrovirus Serotype 1 and Synthesis of Infectious RNA Transcripts”, Feb. 1997, J. Virol., pp. 1713-1717, vol. 71, No. 2, American Society for Microbiology, Washington, DC.
B.C. Horsburgh et al., “Allele Replacement: An Application That Permits Rapid Manipulation of Herpes Simplex Virus Type 1 Genomes”, Gene Therapy, May 1999, pp. 922-930, vol. 6, No. 5, Stockton Press, Hampshire, UK.
B. Hsue et al., “Insertion of a New Transcriptional Unit Into the Genome of Mouse Hepatitis Virus”, J. Virol., Jul. 1999, pp. 6128-6135, vol. 73, No. 7, American Society for Microbiology, Washington, DC.
A. Izeta et al., “Replication and Packaging of Transmissible Gastroenteritis Coronavirus-Derived Synthetic Minigenomes”, J. Virol., Feb. 1999, pp. 1535-1545, vol. 73, No. 2, American Society for Microbiology, Washington, DC.
G. Ketner et al., “Efficient Manipulation of the Human Adenovirus Genome as an Infectious Yeast Artificial Chromosome Clone”, Proc. Natl. Acad. Sci. USA, Jun. 1994, pp. 6186-6190, vol. 91, The National Academy of Sciences, Washington, DC.
U.-J. Kim et al., “Stable Propagation of Cosmid Sized Human DNA Inserts in an F Factor Based Vector”, Nucleic Acids Res., Mar. 11, 1992, pp. 1083-1085, vol. 20, No. 5, Oxford University Press, Oxford, UK.
L. Kuo et al., “Retargeting of Coronavirus by Substitution of the Spike Glycoprotein Ectodomain: Crossing the Host Cell Species Barrier”, J. Virol., Feb. 2000, pp. 1393-1406, vol. 74, No. 3, ASM Press, Washington, DC.
M.C. Lai, “The Making of Infectious Viral RNA: No Size Limit in Sight”, Proc. Natl., Acad. Sci. USA,
Bingham & McCutchen LLP
Chen Stacy B
Consejo Superior de Investigationes Cientificas
Crane Sharon E.
LandOfFree
Bacterial artificial chromosome construct encoding... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Bacterial artificial chromosome construct encoding..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Bacterial artificial chromosome construct encoding... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4034303