Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1996-05-28
1998-07-21
Leary, Louise
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 14, 435 18, 435 21, 435 25, 435 12, 435822, 435 4, 422 50, 422 681, C12Q 104, C12Q 134, C12Q 154
Patent
active
057834105
DESCRIPTION:
BRIEF SUMMARY
FIELD OF THE INVENTION
The invention involves a bacteria-coding identificaton method, which is for parenteral gram-negative bacilli, enteric gram-negative bacilli, Enterobacteriaceae bacteria, anaerobic bacteria, neisseria/hemophilus, campylobacter, yeast-like fungi, corynebacteria, Micrococcaceae bacteria, Streptoccaceae bacteria, bacilli and lactobacilli, and bacteria biochemical properties-identifying papers which detect the biological properties using this method, i.e. identification papers for parenteral gram-negative bacilli, enteric gram-negative bacilli, Enterobacteriaceae bacteria, anaerobic bacteria, neisseria/hemophilis, campylobacter, yeast-like fungi, corgnebacteria, Micrococcaceae bacteria, Streptoccaceae bacteria, bacilli and lactobacilli, and apparatus or means useful for identifying the genus and species of bacteria using the coding identification method and biochemical properties-identifying papers.
BACKGROUND OF THE INVENTION
Bacteria ideatification is a course including detecting the biological properties of the strain to be identificated using the bacteria biological properties assay device, and then according to the detection results of the biological properties of the strain, determining its genus and species by identification method and with the help of identification apparatus or identification coding retrieval means. At present, there are two kinds of bacteria identification met , which are double-branch identification and numerical identification, The former, which is well-known, achieves the identification of genus and species of the stain to be identified by analyzing the detection results of the biological properties one by one. The method contains too many identification steps and costs a lot time because the assay route and the next item of biological property to be detected cannot be determined until the detection result of the former one has been obtained and the steps are repeated again and again until the identification conclusion can be drawn. Meanwhile, the method Can not provide probability parameter when the bacteria identification result is shown because it is logical reasoning analytics. In order to decrease steps and reduce time, scholars abroad put forward numerical identification methods in succession, such as differentiation analysis method, nearest neighbor analysis method, Ochireed distance method, related coefficient method, probability most approximate value model method et. al.
These methods can simultaneously analyse several detection results of the biological properies of the strain to be identifed, which are obtained at one time, and provide probability parameter when the bacteria identification result is shown. Among the methods, probability most approximate value model identification method is applied at most and its effect is beat. Numerical identification methods, including probability most approximate value model method, are also called coding identification methods because in the course of bacteria identification by numerical methods several biological properties of bacteria usually need to be detected and the detection items or detection results need to be coded. There are 15 to 32 coding test items or sorts of test medium in the paper-hole respectively in the well-known coding identification methods or bacteria biochemical properties-identifying papers, such as VITEK, ATB, API, Micro-ID and Entertube II et al. Because of the unreasonable selection and inferior combination effect of the coding test items or the test medium sorts in the hole of bacteria biochemical properties-identifying papers, the identification of the bacteria identified for group is inefficient. Because the amount o coding test items or the medium sorts of biochemical properties-identifying papers is large and pats of coding test items have not identification value to identifying bacteria for group in the combination, the consumption and waste is age.
Different identification apparatus o identification coding retrieval means is used in the course of bacteria identification
REFERENCES:
patent: 4453220 (1984-06-01), Flegal et al.
patent: 4526865 (1985-07-01), Silman
patent: 4767702 (1988-08-01), Cohenford
patent: 5096668 (1992-03-01), Thompson
patent: 5185859 (1993-02-01), Guttag et al.
He Lin
Liang Baoduo
Liu Qiuming
Wang Jinliang
He Lin
Leary Louise
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