Bacteria for expressing a polysaccharide depolymerase containing

Details Bacteria for expressing a polysaccharide depolymerase containing Bacteria for expressing a polysaccharide depolymerase containing

1985-10-07

1989-04-18

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435206, 435320, 435847, 43525233, 935 27, 935 31, 935 73, 424 9461, C12N 924, C12N 936, C12N 120, C12N 1500, C12R 118, A61K 3754

Patent

active

048227404

ABSTRACT:
A segment of a bacteriophage encoding for a polysaccharide depolymerase which has been cloned and expressed in Escherichia coli is described. In particular, bacteriophage ERA103 was found to consist of five EcoRI fragments labeled: A, 7.5-kb; B, 5.0-kb; C, 2.7-kb; D, 2.1-kb and E, 1.8-kb. Fragment B encodes for the depolymerase and was cloned into the positive-selection vector pOP203(A.sub.2.sup.+), pBR322 and the expression vector pKK223-3. The depolymerase is applied to rosaceous plants to prevent Fireblight caused by Erwinia amylovora by depolymerizing a polysaccharide produced by this bacteria.

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