Bacteria expressing nonsecreted cytolysin as intracellular micro

Details Bacteria expressing nonsecreted cytolysin as intracellular micro Bacteria expressing nonsecreted cytolysin as intracellular micro

1998-08-13

1999-12-21

Railey, II, Johnny F.

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

Fusion of cells

4352523, 43525233, 435325, 435373, 4242001, C12N 121, C12N 510, A61K 3900

Patent

active

060048158

ABSTRACT:
The invention provides methods and compositions relating to intracellular delivering of agents to eukaryotic cells. The compositions include microbial delivery vehicles such as nonvirulent bacteria comprising a first gene encoding a nonsecreted foreign cytolysin operably linked to a heterologous promoter and a second gene encoding a different foreign agent. The foreign agent may be a nucleic acid or protein, and is frequently bioactive in and therapeutic to the target eukaryote. In addition, the invention provides eukaryotic cells comprising the subject nonvirulent bacteria and nonhuman eukaryotic host organisms comprising such cells.

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