Bacteria expressing metallothionein gene into the periplasmic sp

Details Bacteria expressing metallothionein gene into the periplasmic sp Bacteria expressing metallothionein gene into the periplasmic sp

1996-11-22

1998-10-20

Carlson, Karen Cochrane

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

43525233, 4352523, 530350, 536 234, C12P 2106, C12N 120, C07H 1700, C07K 1400

Patent

active

058245120

ABSTRACT:
A gene coding for the expression of metallothionein is incorporated into an xpression vehicle for expressing the metallothionein gene into the periplasmic space of a bacterium including the expression vehicle. It may also be possible to incorporate multiple copies of the metallothionein gene within a single bacterium. One typical expression vehicle is a p-MalP plasmid constructed to include the metallothionein gene. Using this expression vehicle, one can induce bacteria, such as transformed E. coli, to express metallothionein into the periplasmic space as a fusion product with, for example, the maltose-binding protein. Killed cell that have expressed metallothionein into the periplasmic space may then be attached to a solid support. The resulting device may be used to remove heavy metals from aqueous media.

REFERENCES:
patent: 5500353 (1996-03-01), Smit et al.
patent: 5516697 (1996-05-01), Kruzel
Pazirandeh et al. Dec. 1995 43(6):1112-1117.
Jacobs et al. 1989 Gene 83:95-103.
Maina et al. 1988 Gene 74:365-373.

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