Bacteria counting method

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

Reexamination Certificate

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Reexamination Certificate

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07422870

ABSTRACT:
Methods for counting bacteria are described that include: (a) preparing an assay sample by staining a specimen using a fluorescent dye, thereby producing a difference in fluorescent intensity between live bacteria and dead bacteria; (b) detecting optical information from the assay sample; and (c) classifying and counting the live bacteria and the dead bacteria based on the detected optical information. Bacteria counting apparatuses and reagent kits for counting bacteria are also described.

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Roth, Bruce L.; Poot, Martin; Yue, Stephen T.; Millard, Paul J. “Bacterial Viability and Antibiotic Susceptibility Testing with SYTOX Green Nucleic Acid Stain”,Applied and Environmental Microbiology, 1997, 63, 2421-2431.
Suller, M.T.E.; Lloyd, D. “Fluorescence Monitoring of Antibiotic-Induced Bacterial Damage Using Flow Cytometry”,Cytometry, 1999, 35, 235-241.
XP-002270007 Abstract of SU 1306947 A, V.S. Bannikov, Apr. 30, 1987.

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