Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1998-06-17
1999-03-30
Lankford, Jr., Leon B.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
53612312, 435 72, 435 98, 514 61, C07H 100
Patent
active
058891790
ABSTRACT:
A new enzyme, alternanase, which is effective for the endo-hydrolytic cleavage of alternan, producing a thinned composition of low molecular weight fractions which exhibit reduced viscosity and increased solubility relative to native alternan, is described. The enzyme is produced and secreted extracellularly by a plurality of novel bacteria isolated from soil. One of the fractions present in the thinned alternan resulting from hydrolysis with alternanase is a the cyclic tetrasaccharide, cyclo{-6)-.alpha.-D-Glcp-(1,3)-.alpha.-D-Glcp-(1,6)-.alpha.-D-Glcp-(1,3)-. alpha.-D-Glcp-(1-}. A novel method for isolating strains of microorganisms which produce endo-.alpha.-D-glucanases such as alternanase effective for the endo-hydrolytic cleavage or thinning of alternan is also described. Cultures of the subject strains are contacted with a test substrate of alternan coupled to a detectable indicator. Detection of released indicator provides an indication of endo-.alpha.-D-glucanase activity.
Cote Gregory L.
Wyckoff Herbert
Deck Randall E.
Fado John D.
Lankford , Jr. Leon B.
Silverstein M. Howard
The United States of America as represented by the Secretary of
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