Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-06-12
1998-07-28
Lankford, Jr., Leon B.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435195, 435200, 435210, C12N 940, C12N 944
Patent
active
057861960
ABSTRACT:
A new enzyme, alternanase, which is effective for the endo-hydrolytic cleavage of alternan, producing a thinned composition of low molecular weight fractions which exhibit reduced viscosity and increased solubility relative to native alternan, is described. The enzyme is produced and secreted extracellularly by a plurality of novel bacteria isolated from soil. One of the fractions present in the thinned alternan resulting from hydrolysis with alternanase is a the cyclic tetrasaccharide, cyclo{-6)-.alpha.-D-Glcp-(1,3)-.alpha.-D-Glcp-(1,6)-.alpha.-D-Glcp-(1,3)-. alpha.-D-Glcp-(1-}. A novel method for isolating strains of microorganisms which produce endo-.alpha.-D-glucanases such as alternanase effective for the endo-hydrolytic cleavage or thinning of alternan is also described. Cultures of the subject strains are contacted with a test substrate of alternan coupled to a detectable indicator. Detection of released indicator provides an indication of endo-.alpha.-D-glucanase activity.
REFERENCES:
Biely, Peter, et al., "Purification and Properties of alternanase, a novel endo-.alpha.-1,3.alpha.-,6-D-glucanase", Eur. J. Biochem., 226, 1994, pp. 633-639.
Cote, Greogy L., et al., "Enzymically produced cyclic .alpha.-1, 3-linked and .alpha.-1,6-linked oligosaccharides of D-glucose", Eur. J. Biochem., 1994, pp. 641-648.
Cote, Gregory L. and Biely, Peter, "Enzymatically Produced Cyclic .alpha.(1.fwdarw.3), .alpha.(1.fwdarw.6) Linked Oligosaccharides of D-Glucose", Abstract, XVIIth International Carbohydrate Symposium, Ottawa, Canada, Jul. 17-22, 1994, p. 397.
Biely, P, et al., "Purification and Properties of Alternanase--A New Type of .alpha.-1,3-.alpha.-1,6-Glucanase", Abstract, Programme and Abstracts, 7th Bratislava Symposium on Saccharides, Smolenice, Slovakia, Aug. 29-Sep. 2, 1994, p. 44.
Cote, Greogy L., et al., Enzymatically Produced Cyclic .alpha.(1.fwdarw.3) and .alpha.(1.fwdarw.6) Linked Oligosaccharides of D-Glucose, Abstract, Programme and Abstracts, 7th Bratislava Symposium on Saccharides, SMolenice, Slovakia, Aug. 29-Sep. 2, 1994, p. 45.
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Cote, Gregory and Robyt, John F., "Isolation and Partial Characterization of an Extracellular Glucansucrase from Leuconostoc mesenteroides NRRL-B-1355 That Synthesizes an Alternating (1.fwdarw.6),(1.fwdarw.3)-.alpha.-D-Glucan", Carbohydrate Research, 101, 1982, pp. 57-74.
Sawai, Teruo, et al., "Purification and Some Properties of the Isomaltodextranase of Actinomadura Strain R10 and Comparison with that of Arthrobacter globiformis T6", Carbohydrate Research, 89, 1981, pp. 1289-299.
Sawai, Teruo, et al., "Hydrolysis of Fourteen Native Dextrans by Arthrobacter Isomaltodextranase and Correlation with Dextran Structure", Carbohydrate Research, 66, 1978, pp. 195-205.
Cote, Gregory L., "Low-viscosity .alpha.-D-glucan fractions derived from sucrose which are resistant to enzymatic digestion", Carbohydrate Polymers, 19, 1992, pp. 249-252.
Misaki, Akira, et al., "Structure of the Dextran of Leuconostoc mesenteroides B-1355", Carbohydrate Research, 84, 1980, pp. 273-285.
Biely Peter
Cote Gregory L.
Wyckoff Herbert
Deck Randall E.
Fado John D.
Lankford , Jr. Leon B.
Silverstein M. Howard
The United States of America as represented by the Secretary of
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