Back-scatter detection in flow cytometers

Optics: measuring and testing – By particle light scattering – With photocell detection

Reexamination Certificate

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Reexamination Certificate

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07075647

ABSTRACT:
A method and apparatus for detecting back-scattered light from small particles, such as blood cells, irradiated by a focused beam of light as such particles travel through the interrogation zone of a standard optical flow cell in a flow cytometer. Back-scattered light is collected at a location upstream of the beam-shaping optics (or a portion of such optics), at a location between the light beam source and the beam-shaping optics.

REFERENCES:
patent: 5125737 (1992-06-01), Rodriguez et al.
patent: 6177277 (2001-01-01), Soini
patent: 6228652 (2001-05-01), Rodriguez et al.
patent: 2004/0036874 (2004-02-01), Kramer
patent: 2004/0036875 (2004-02-01), Kramer

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