Bacillus thuringiensis strains and their insecticidal proteins

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

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800DIG55, 800DIG56, 435 691, 4351723, 4353201, 435419, 536 2371, C12N 1532, C12N 1582, A01H 400, A01H 500

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060282460

ABSTRACT:
Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as spodoptera spp. and Agotis ipsilon, against Pyralidae such as Ostrinia nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains nd that encodes its respective toxin, is resistant to Lipidoptera. Each strain, itself, or its crystal, crystal protein, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combating Lepidoptera.

REFERENCES:
Smulevitch et al., FEBS Letters, 298, 25-28 (1991).
Vaeck et al., Nature, 328, 33-37 (1987).
Hofte et al., Microbiological Reviews, 53, 242-255 (1989).
Gleave et al. Journal of General Microbiology 138: 55-62 1992 Identification of an Insecticidal Crystal Protein from Bacillus . . . .
Perlak et al. Bio/Technology vol. 8: 939-943 Oct. 1990 Insect Resistant Cotton Plants.
Perlak et al. Proc. Natl. Acad. Sci. USA vol. 88: 3324-3328 Apr. 1991 Modification of The Coding Sequence Enhances Plant Expression . . . .

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